研究業績

2023

Lee CE, Ikeda JH, Manongdo MAM, Romerosa DRT, Sandalo-De Ramos KAC, Tanaka T, Galay RL. Molecular detection of Bartonella and Borrelia in pet dogs in Metro Manila and Laguna, Philippines. Vet World. 16: 1546-1551. Jul (2023) pubmed
Ali A, Obaid MK, Almutairi MM, Alouffi A, Numan M, Ullah S, Rehman G, Islam ZU, Khan SB, Tanaka T. Molecular detection of Coxiella spp. in ticks (Ixodidae and Argasidae) infesting domestic and wild animals: with notes on the epidemiology of tick-borne Coxiella burnetii in Asia. Front Microbiol. 14: 1229950. Jul (2023) pubmed
Kwofie KD, Hernandez EP, Anisuzzaman, Kawada H, Koike Y, Sasaki S, Inoue T, Jimbo K, Mikami F, Ladzekpo D, Umemiya-Shirafuji R, Yamaji K, Tanaka T, Matsubayashi M, Alim MA, Dadzie SK, Iwanaga S, Tsuji N, Hatta T. RNA activation in ticks. Sci Rep. 13: 9341. Jun (2023) pubmed
Mahmoud HYAH, Ali AO, Tanaka T. Molecular detection and characterization of Anaplasma marginale infecting cattle, buffalo, and camel populations in southern Egypt. Front Vet Sci. 10: 1169323. May (2023) pubmed
Obaid MK, Almutairi MM, Alouffi A, Safi SZ, Tanaka T, Ali A. Assessment of cypermethrin and amitraz resistance and molecular profiling of voltage-gated sodium channel and octopamine tyramine genes of Rhipicephalus microplus. Front Cell Infect Microbiol.13: 1176013. May (2023) pubmed
Ullah S, Alouffi A, Almutairi MM, Islam N, Rehman G, Ul Islam Z, Ahmed H, Júnior IDSV, Labruna MB, Tanaka T, Ali A. First Report of Rickettsia conorii in Hyalomma kumari Ticks. Animals (Basel) 13: 1488. Apr (2023) pubmed
Feng C, Torimaru K, Lim MYT, Chak LL, Shiimori M, Tsuji K, Tanaka T, Iida J, Okamura K. A novel eukaryotic RdRP-dependent small RNA pathway represses antiviral immunity by controlling an ERK pathway component in the black-legged tick. PLoS One 18: e0281195. Mar (2023) pubmed
Hifumi T, Tanaka T, Sato M, Akioka K, Fujimata C, Miyoshi N. Rapid detection of alveolar echinococcosis in hepatic nodules of horses by recombinase polymerase amplification assay. Vet Anim Sci. 20: 100291. Mar (2023) pubmed
Hernandez EP, Shimazaki K, Niihara H, Umemiya-Shirafuji R, Fujisaki K, Tanaka T. Localization of secreted ferritin (FER2) in the embryos of the tick Haemaphysalis longicornis. Parasit Vectors 16: 42. Jan (2023) pubmed
Matsuu A, Hatai H, Hifumi T, Hamakubo E, Take M, Tanaka T, Momoi Y, Endo Y, Koyoshi A, Kamikubo Y, Kamatsuki R, Kurusu N, Tabata H, Matsuyama H, Yanaidani M, Kawabata M, Kawabata T. Clinical and Pathological Findings in Fatal Cases of Severe Fever With Thrombocytopenia Syndrome With High Viremia in Cats. Top Companion Anim Med. 52: 100756. Jan (2023) pubmed
Khan SM, Khan M, Alouffi A, Almutairi MM, Numan M, Ullah S, Obaid MK, Islam ZU, Ahmed H, Tanaka T, Ali A. Phylogenetic Position of Haemaphysalis kashmirensis and Haemaphysalis cornupunctata, with Notes on Rickettsia spp. Genes (Basel) 14: 360. Jan (2023) pubmed

2022

Ahmad I, Ullah S, Alouffi A, Almutairi MM, Khan M, Numan M, Safi SZ, Chitimia-Dobler L, Tanaka T, Ali A. Description of Male, Redescription of Female, Host Record, and Phylogenetic Position of Haemaphysalis danieli. Pathogens 11: 1495. Dec (2022) pubmed
Zeb I, Almutairi MM, Alouffi A, Islam N, Parizi LF, Safi SZ, Tanaka T, da Silva Vaz I Jr, Ali A. Low Genetic Polymorphism in the Immunogenic Sequences of Rhipicephalus microplus Clade C. Vaccines (Basel) 10: 1909. Nov (2022) pubmed
Ullah K, Numan M, Alouffi A, Almutairi MM, Zahid H, Khan M, Islam ZU, Kamil A, Safi SZ, Ahmed H, Tanaka T, Ali A. Molecular Characterization and Assessment of Risk Factors Associated with Theileria annulata Infection. Microorganisms 9: 1614. Aug (2022) pubmed
Alam S, Khan M, Alouffi A, Almutairi MM, Ullah S, Numan M, Islam N, Khan Z, Aiman O, Zaman Safi S, Tanaka T, Ali A. Spatio-Temporal Patterns of Ticks and Molecular Survey of Anaplasma marginale, with Notes on Their Phylogeny. Microorganisms 17: 1663. Aug (2022) pubmed
Obaid MK, Islam N, Alouffi A, Khan AZ, da Silva Vaz I Jr, Tanaka T, Ali A. Acaricides Resistance in Ticks: Selection, Diagnosis, Mechanisms, and Mitigation. Front Cell Infect Microbiol. 6: 941831. Jul (2022) pubmed
Khan Z, Shehla S, Alouffi A, Kashif Obaid M, Zeb Khan A, Almutairi MM, Numan M, Aiman O, Alam S, Ullah S, Zaman Safi S, Tanaka T, Ali A. Molecular Survey and Genetic Characterization of Anaplasma marginale in Ticks Collected from Livestock Hosts in Pakistan. Animals (Basel) 12: 1708. Jul (2022) pubmed
Senbill H, Tanaka T, Karawia D, Rahman S, Zeb J, Sparagano O, Baruah A. Morphological identification and molecular characterization of economically important ticks (Acari: Ixodidae) from North and North-Western Egypt. Acta Trop. 231. Jul (2022) pubmed
Hernandez EP, Anisuzzaman, Alim MA, Kawada H, Kwofie KD, Ladzekpo D, Koike Y, Inoue T, Sasaki S, Mikami F, Matsubayashi M, Tanaka T, Tsuji N, Hatta T. Ambivalent Roles of Oxidative Stress in Triangular Relationships among Arthropod Vectors, Pathogens and Hosts. Antioxidants (Basel) 25: 1254. Jun (2022) pubmed
Ali A, Zeb I, Alouffi A, Zahid H, Almutairi MM, Ayed Alshammari F, Alrouji M, Termignoni C, Vaz IDS Jr, Tanaka T. Host Immune Responses to Salivary Components - A Critical Facet of Tick-Host Interactions. Front Cell Infect Microbiol. 16: 12. Mar (2022) pubmed
Prado ICB, Capuno LXB Jr, Collera PD, Cabralda APD, De Ramos KAS, Bernardo JMG, Divina BP, Masatani T, Tanaka T, Galay RL. Molecular Detection and Characterization of Babesia and Theileria in Cattle and Water Buffaloes from Southern Luzon, Philippines. Microorganisms 10: 678. Mar (2022) pubmed
Zhang X, Zhao C, Cheng C, Zhang G, Yu T, Lawrence K, Li H, Sun J, Yang Z, Ye L, Chu H, Wang Y, Han X, Jia Y, Fan S, Kanuka H, Tanaka T, Jenkins C, Gedye K, Chandra S, Price DC, Liu Q, Choi YK, Zhan X, Zhang Z, Zheng A. Rapid Spread of Severe Fever with Thrombocytopenia Syndrome Virus by Parthenogenetic Asian Longhorned Ticks. Emerg Infect Dis. 28: 363-372. Feb (2022) pubmed
Ali A, Shehla S, Zahid H, Ullah F, Zeb I, Ahmed H, da Silva Vaz I Jr, Tanaka T. Molecular Survey and Spatial Distribution of Rickettsia spp. in Ticks Infesting Free-Ranging Wild Animals in Pakistan (2017-2021). Pathogens 11: 162. Jan (2022) pubmed
Hendawy SHM, Alzan HF, Tanaka T, Mahmoud MS. Fundamental Tick Vaccinomic Approach to Evade Host Autoimmune Reaction. Methods Mol Biol. 2411: 343-358. Jan (2022) pubmed
Kusakisako K, Miyata T, Fujisaki K, Tanaka T. Host Immunization with Recombinant Tick Antigen and Evaluation of Host Immune Response. Methods Mol Biol. 2411: 331-341. Jan (2022) pubmed

2021

Galay RL, Llaneta CR, Monreal MKFB, Armero AL 3rd, Baluyut ABD, Regino CMF, Sandalo KAC, Divina BP, Talactac MR, Tapawan LP, Mojares MCL, Alvarez CR, Mago ER, Encarnacion ND, Andoh M, Tanaka T. Molecular Prevalence of Anaplasma marginale and Ehrlichia in Domestic Large Ruminants and Rhipicephalus (Boophilus) microplus Ticks From Southern Luzon, Philippines. Front Vet Sci. 13: 8. Oct (2021) pubmed
Hifumi T, Akioka K, Tanaka T, Miyoshi N. Development of a loop-mediated isothermal amplification (LAMP) assay targeting the mitochondrial cytochrome b gene for the rapid detection of alveolar echinococcosis in hepatic nodules of horses. Vet Parasitol. 299:109573. Sep (2021) pubmed
Futagami T, Mori K, Kadooka C, Niihara H, Tashiro K, Tamaki H, Tanaka T. Chromosome-Level Genome Sequence of Aspergillus puulaauensis MK2, a Fungus Isolated from a Dead Hard Tick. Microbiol Resour Announc. 9: 10. Sep (2021) pubmed
Talactac MR, Hernandez EP, Hatta T, Yoshii K, Kusakisako K, Tsuji N, Tanaka T. The antiviral immunity of ticks against transmitted viral pathogens. Dev Comp Immunol. 119. Jun (2021) pubmed
Alota SL, Edquiban TRJ, Galay RL, Bernardo JMG, Sandalo KAC, Divina BP, Tanaka T. Determination of resistance status to amitraz in the cattle tick Rhipicephalus (Boophilus) microplus from Luzon, Philippines, through bioassay and molecular analysis. Exp Appl Acarol. 83: 399-409. Mar (2021) pubmed
Hernandez EP, Talactac MR, Vitor RJS, Yoshii K, Tanaka T.
An Ixodes scapularis glutathione S-transferase plays a role in cell survival and viability during Langat virus infection of a tick cell line. Acta Trop. 214: 105763. Feb (2021) pubmed

2020

Chua APB, Galay RL, Tanaka T, Yamazaki W.
Development of a Loop-Mediated Isothermal Amplification (LAMP) assay targeting the citrate synthase gene for detection of Ehrlichia canis in dogs. Vet Sci. 7: 156. Oct (2020) pubmed
Galay RL, Talactac MR, Ambita-Salem BV, Chu DMM, Costa LMOD, Salangsang CMA, Caracas DKB, Generoso FH, Babelonia JA, Vergano JL, Berana LC, Sandalo KAC, Divina BP, Alvarez CR, Mago ER, Andoh M, Tanaka T.
Molecular detection of Rickettsia spp. and Coxiella burnetii in cattle, water buffalo, and Rhipicephalus (Boophilus) microplus ticks in Luzon island of the Philippines. Trop Med Infect Dis. 5: 54. Apr (2020) pubmed
Hernandez EP, Shimazaki K, Niihara H, Umemiya-Shirafuji R, Fujisaki K, Tanaka T.
Expression analysis of glutathione S-transferases and ferritins during the embryogenesis of the tick Haemaphysalis longicornis.
Heliyon 6: e03644. Mar (2020) pubmed
Kusakisako K, Morokuma H, Talactac MR, Hernandez EP, Yoshii K, Tanaka T.
A peroxiredoxin from the Haemaphysalis longicornis tick affects Langat virus replication in a hamster cell line.
Front Cell Infect Microbiol. 10: 7. Jan (2020) pubmed

2019

Dela CA, Galay RL, Sandalo KAC, Pilapil-amante FMIR, Tanaka T.
Molecular detection of Anaplasma spp. in blood and milk in dairy cattle in the Philippines.
Turk J Vet Anim Sci. 43: 540-545. Jul (2019) PDF
Hernandez EP, Kusakisako K, Hatta T, Tanaka T.
Characterization of an iron-inducible Haemaphysalis longicornis tick-derived promoter in an Ixodes scapularis-derived tick cell line (ISE6).
Parasit Vectors 12: 321. Jun (2019) pubmed
Hernandez EP, Talactac MR, Fujisaki K, Tanaka T.
The case for oxidative stress molecule involvement in the tick-pathogen interactions -an omics approach.
Dev Comp Immunol. 100: 103409. Jun (2019) pubmed

2018

Talactac MR, Hernandez EP, Fujisaki K, Tanaka T.
A continuing exploration of tick–virus interactions using various experimental viral infections of hard ticks.
Front Physiol. 9: 1728. Dec (2018) pubmed
Galay RL, Manalo AA, Dolores SL, Aguilar IP, Sandalo KA, Cruz K, Divina B, Andoh M, Masatani T, Tanaka T.
Molecular detection of tick-borne pathogens in canine population and Rhipicephalus sanguineus (sensu lato) ticks from southern Metro Manila and Laguna, Philippine.
Parasit Vectors 11: 643. Dec (2018) pubmed
Hernandez EP, Kusakisako K, Talactac MR, Galay RL, Yoshii K, Tanaka T.
Induction of intracellular ferritin expression in embryo-derived Ixodes scapularis cell line (ISE6).
Sci Rep. 8: 16566. Nov (2018) pubmed
Kusakisako K, Ido A, Masatani T, Morokuma H, Hernandez EP, Talactac MR, Yoshii K, Tanaka T.
Transcriptional activities of two newly identified Haemaphysalis longicornis tick-derived promoter regions in the Ixodes scapularis tick cell line (ISE6).
Insect Mol Biol. 27: 590-602. Oct (2018) pubmed
Hernandez EP, Kusakisako K, Talactac MR, Galay RL, Hatta T, Fujisaki K, Tsuji N, Tanaka T.
Glutathione S-transferases play a role in the detoxification of flumethrin and chlorpyrifos in Haemaphysalis longicornis.
Parasit Vectors 11: 460. Aug (2018) pubmed
Kusakisako K, Fujisaki K, Tanaka T.
The multiple roles of peroxiredoxins in tick blood feeding.
Exp Appl Acarol. 75: 269-280. Jul (2018) pubmed
Yada Y, Talactac MR, Kusakisako K, Hernandez EP, Galay RL, Andoh M, Fujisaki K, Tanaka T.
Hemolymph defensin from the hard tick Haemaphysalis longicornis attacks Gram-positive bacteria.
J Invertebr Pathol. 156: 14-18. Jul (2018) pubmed
Talactac MR, Yoshii K, Hernandez EP, Kusakisako K, Galay RL, Fujisaki K, Mochizuki M, Tanaka T.
Vector competence of Haemaphysalis longicornis ticks for a Japanese isolate of the Thogoto virus.
Sci Rep. 8: 9300. Jun (2018) pubmed
Kusakisako K, Hernandez EP, Talactac MR, Yoshii K, Umemiya-Shirafuji R, Fujisaki K, Tanaka T.
Peroxiredoxins are important for the regulation of hydrogen peroxide concentrations in ticks and tick cell line.
Ticks Tick Borne Dis. 9: 872-881. Mar (2018) pubmed
Hernandez EP, Kusakisako K, Talactac MR, Galay RL, Hatta T, Matsuo T, Fujisaki K, Tanaka T.
Characterization and expression analysis of a newly identified glutathione S-transferase of the hard tick Haemaphysalis longicornis during blood feeding.
Parasit Vectors 11: 91. Feb (2018) pubmed
Kusakisako K, Miyata T, Tsujio M, Galay RL, Talactac MR, Hernandez EP, Fujisaki K, Tanaka T.
Evaluation of vaccine potential of 2-Cys peroxiredoxin from the hard tick Haemaphysalis longicornis.
Exp Appl Acarol. 74: 73-84. Jan (2018) pubmed

2017

Galay RL, Matsuo T, Hernandez RP, Talactac MR, Kusakisako K, Umemiya-Shirafuji R, Fujisaki K, Tanaka T.
Immunofluorescent detection in the ovary of host antibodies against a secretory ferritin injected into female Haemaphysalis longicornis ticks.
Parasitol Int. 67: 119-122. Nov (2017) pubmed
Talactac MR, Yoshii K, Hernandez EP, Kusakisako K, Galay RL, Fujisaki K, Mochizuki M, Tanaka T.
Synchronous Langat virus infection of Haemaphysalis longicornis using anal pore microinjection.
Viruses 9: 189. Jul (2017) pubmed
Masatani T, Hayashi K, Andoh M, Tateno M, Endo Y, Asada M, Kusakisako K, Tanaka T, Gokuden M, Hozumi N, Nakadohzono F, Matsuo T.
Detection and molecular characterization of Babesia, Theileria, and Hepatozoon species in hard ticks collected from Kagoshima, the southern region in Japan.
Ticks Tick Borne Dis. 8: 581-587. Jun (2017) pubmed
Talactac MR, Yada Y, Yoshii K, Hernandez EP, Kusakisako K, Hiroki M, Galay RL, Fujisaki K, Mochizuki M, Tanaka T.
Characterization and antiviral activity of a newly identified defensin-like peptide, HEdefensin, in the hard tick Haemaphysalis longicornis.
Dev Comp Immunol. 68: 98-107. Mar (2017) pubmed
Galay RL, Hernandez EP, Talactac MR, Kusakisako K, Fujisaki K and Tanaka T.
Ticks' Antioxidant Complex: A Defense Stronghold and a Potential Target for Their Control, Advances in Medicine and Biology Volume 116. (Editors: Leon V. Berhardt)
Nova Science Publishers 116: 231-256. Jan (2017) Book
Maeda H, Hatta T, Alim MA, Tsubokawa D, Mikami F, Kusakisako K, Matsubayashi M, Umemiya-Shirafuji R, Tsuji N, Tanaka T.
Initial development of Babesia ovata in the tick midgut.
Vet Parasitol. 15: 39-42. Jan (2017) pubmed

2016

Maeda H, Hatta T, Alim MA, Tsubokawa D, Mikami F, Matsubayashi M, Miyoshi T, Umemiya-Shirafuji R, Kawazu SI, Igarashi I, Mochizuki M, Tsuji N, Tanaka T.
Establishment of a novel tick-Babesia experimental infection model.
Sci Rep. 6: 37039. Nov (2016) pubmed
Kusakisako K, Masatani T, Yada Y, Talactac MR, Hernandez EP, Maeda H, Mochizuki M, Tanaka T.
Improvement of the cryopreservation method for the Babesia gibsoni parasite by using commercial freezing media.
Parasitol Int. 65: 532-535. Oct (2016) pubmed
Kusakisako K, Galay RL, Umemiya-Shirafuji R, Hernandez EP, Maeda H, Talactac MR, Tsuji N, Mochizuki M, Fujisaki K, Tanaka T.
2-Cys peroxiredoxin is required in successful blood-feeding, reproduction, and antioxidant response in the hard tick Haemaphysalis longicornis.
Parasit Vectors 9: 457. Aug (2016) pubmed
Galay RL, Hernandez EP, Talactac MR, Maeda H, Kusakisako K, Umemiya-Shirafuji R, Mochizuki M, Fujisaki K, Tanaka, T.
Induction of gene silencing in Haemaphysalis longicornis ticks through immersion in double-stranded RNA.
Ticks Tick Borne Dis. 7: 813–816. Jul (2016) pubmed
Masatani T, Yoshihara S, Matsubara A, Gotoh T, Takahashi H, Tanaka T, Andoh M, Endo Y, Matsuo T.
Dynamics of Theileria orientalis genotype population in cattle in a year-round grazing system.
Acta Parasitol. 61: 419-424. Jun (2016) pubmed
Galay RL, Takechi R, Umemiya-Shirafuji R, Talactac MR, Maeda H, Kusakisako K, Mochizuki M, Fujisaki K, Tanaka T.
Impaired cellular immune response to injected bacteria after knockdown of ferritin genes in the hard tick Haemaphysalis longicornis.
Parasitol Int. 65: 251-257. Jun (2016) pubmed
Maeda H, Miyata T, Kusakisako K, Galay RL, Talactac MR, Umemiya-Shirafuji R, Mochizuki M, Fujisaki K, Tanaka T.
A novel C-type lectin with triple carbohydrate recognition domains has critical roles for the hard tick Haemaphysalis longicornis against Gram-negative bacteria.
Dev Comp Immunol. 57: 38-47. Apr (2016) pubmed
Takechi R, Galay RL, Maeda H, Matsuo T, Kusakisako K, Talactac MR, Mochizuki M, Fujisaki K, Tanaka T.
Role of the tumor necrosis factor receptor-associated factor-type zinc finger domain containing protein 1 (TRAFD1) from the hard tick Haemaphysalis longicornis in immunity against bacterial infection.
World Biomedical Frontiers (Infection and Immunity) Mar-Apr: 23 (2016) article
Galay RL, Umemiya-Shirafuji R, Mochizuki M, Fujisaki K, Tanaka T.
RNA Interference: A Powerful Functional Analysis Tool for Studying Tick Biology Towards Tick and Pathogen Control, RNA Interference (Editors: Ibrokhim Y. Abdurakhmonov).
InTech-Open Access Publisher pp. 411-445. Apr (2016) article
Galay RL, Miyata T, Umemiya-Shirafuji R, Mochizuki M, Fujisaki K, Tanaka T.
Host immunization with recombinant proteins to screen antigens for tick control, Vaccine Design: Methods and Protocols (Editors: Sunil Thomas).
Springer New York pp. 261-273. Apr (2016) pubmed
Talactac MR, Yoshii K, Maeda H, Kusakisako K, Hernandez EP, Tsuji N, Fujisaki K, Galay RL, Tanaka T, Mochizuki M.
Virucidal activity of Haemaphysalis longicornis longicin P4 peptide against tick-borne encephalitis virus surrogate Langat virus.
Parasit Vectors 9: 59. Feb (2016) pubmed
Takechi R, Galay RL, Maeda H, Matsuo T, Kusakisako K, Talactac MR, Mochizuki M, Fujisaki K, Tanaka T.
Role of the tumor necrosis factor receptor-associated factor-type zinc finger domain containing protein 1 (TRAFD1) from the hard tick Haemaphysalis longicornis in immunity against bacterial infection.
Ticks Tick Borne Dis. 7: 36-45. Feb (2016) pubmed
Kusakisako K, Masatani T, Miyata T, Galay RL, Maeda H, Talactac MR, Tsuji N, Mochizuki M, Fujisaki K, Tanaka T.
Functional analysis of recombinant 2-Cys peroxiredoxin from the hard tick Haemaphysalis longicornis.
Insect Mol Biol. 25: 16-23. Feb (2016) pubmed
Ono U, Matsubayashi M, Tsujio M, Mizuno M, Tanaka T, Masatani T, Matsui T, Matsuo T.
Course of induced infection by Eimeria krijgsmanni in immunocompetent and immunodeficient mice.
Parasitol Res. 115: 211-215. Jan (2016) pubmed

2015

Kusakisako K, Maeda H, Galay RL, Matsuo T, Tsujio M,　Umemiya-Shirafuji R, Mochizuki M, Fujisaki K, Tanaka T.
The vector potential of Haemaphysalis longicornis ticks for Babesia microti parasites under experimental condition.
J Protozool Res.25: 8-17. (2015) PDF
Maeda H, Kurisu K, Miyata T, Kusakisako K, Galay RL, Talactac MR,　Mochizuki M, Fujisaki K, Tanaka T.
Identification of the Babesia-responsive leucine-rich repeat domain-containing protein from the hard tick Haemaphysalis longicornis.
Parasitol Res. 114: 1793-1802. May (2015) pubmed
Galay RL, Umemiya-Shirafuji R, Mochizuki M, Fujisaki K, Tanaka T.
Iron metabolism in hard ticks (Acari: Ixodidae): The antidote to their toxic diet.
Parasitol Int. 64: 182-189. Apr (2015) pubmed
Mori H, Tanaka T, Mochizuki M.
The widely distributed hard tick, Haemaphysalis longicornis, can retain canine parvovirus but not be infected in laboratory condition.
J Vet Med Sci. 77: 405-411. Apr (2015) pubmed
Soma T, Nakagomi O, Nakagomi T, Mochizuki M.
Detection of norovirus and sapovirus from diarrheic dogs and cats in Japan.
Microbiol Immunol. 59: 123-128. Mar (2015) pubmed
Gauchan P, Sasaki E, Nakagomi T, Do LP, Doan YH, Mochizuki M, Nakagomi O.
Whole genotype constellation of prototype feline rotavirus strains FRV-1 and FRV64 and their phylogenetic relationships with feline-like human rotavirus strains.
J Gen Virol. 96: 338-350. Feb (2015) pubmed

2014

Galay RL, Miyata T, Umemiya-Shirafuji R, Maeda H, Kusakisako K, Tsuji N, Mochizuki M, Fujisaki K,Tanaka T.
Evaluation and comparison of the potential of two ferritins as anti-tick vaccines against Haemaphysalis longicornis.
Parasit Vectors 7: 482. Oct (2014) pubmed
Terada Y, Matsui N, Noguchi K, Kuwata R, Shimoda H, Soma T, Mochizuki M, Maeda K.
Emergence of pathogenic coronaviruses in cats by homologous recombination between feline and canine coronaviruses.
PLoS One 9: e106534. Sep (2014) pubmed
Takeo T, Tanaka T, Matsubayashi M, Maeda H, Kusakisako K, Matsui T, Mochizuki M, Matsuo T.
Molecular and phylogenetic characterizations of an Eimeria krijgsmanni Yakimoff & Gouseff, 1938 (Apicomplexa: Eimeriidae) mouse intestinal protozoan parasite by partial 18S ribosomal RNA gene sequence analysis.
Parasitol Int. 63: 627-630. Aug (2014) pubmed
Hashimoto K, Tanaka T, Matsubayashi M, Endo K, Umemiya-Shirafuji R, Matsui T, Matsuo T.
Host specificity and in vivo infectivities of the mouse coccidian parasites Eimeria krijgsmanni.
Acta Parasitol. 59: 337-342. Jun (2014) pubmed
Umemiya-Shirafuji R, Galay RL, Maeda H, Kawano S, Tanaka T, Fukumoto S, Suzuki H, Tsuji N, Fujisaki K.
Expression analysis of autophagy-related genes in the hard tick Haemaphysalis longicornis.
Vet Parasitol. 201: 169-175. Mar (2014) pubmed
Galay RL, Umemiya-Shirafuji R, Bacolod ET, Maeda H, Kusakisako K, Koyama J, Tsuji N, Mochizuki M, Fujisaki K, Tanaka T.
Two kinds of ferritin protect ixodid ticks from iron overload and consequent oxidative stress.
PLoS One 9: e90661. Mar (2014) pubmed
Mori H, Galay RL, Maeda H, Matsuo T, Umemiya-Shirafuji R, Mochizuki M, Fujisaki K, Tanaka T.
Host-derived transferrin is maintained and transferred from midgut to ovary in Haemaphysalis longicornis ticks.
Ticks Tick Borne Dis. 5: 121-126. Mar (2014) pubmed
Hwang CC, Mochizuki M, Maeda K, Okuda M, Mizuno T.
Seroepidemiology of reovirus infection in healthy dogs in six prefectures in Japan.
J Vet Med Sci. 76: 471-475. Mar (2014) pubmed
Terada Y, Noguchi K, Mahmoud HYAH, Shimoda H, Mochizuki M, Une Y, Maeda K.
Genetic characterization of coronaviruses from domestic ferrets in Japan.
Emer Infect Dis. 20: 284-287. Feb (2014) pubmed
Andoh K, Hattori S, Mahmoud HYAH, Takasugi M, Shimoda H, Bannai H, Tsujimura K, Matsumura T, Kondo T, KIrisawa R, Mochizuki M, Maeda K.
The hemagglutination activity of equine herpesvirus type 1 glycoprotein C.
Virus Res. 195: 172-176. Jan (2014) pubmed

2013

Tanaka T, Fujisaki K.
Tick lysozymes.
Microbial pathogens and strategies for combating them: science,technology and education (Editors: A. Mendez-Vilas)
InTech-Open Access Publisher 13: 744-750 (2013) PDF
Hwang CC, Umeki S, Kubo M, Hayashi T, Shimoda H, Mochizuki M, Maeda K, Baba K, Hiraoka H, Coffey M, Okuda M, Mizuno T.
Oncolytic reovirus in canine mast cell tumor.
PLoS One 8: e73555. (2013) pubmed
Masatani T, Matsuo T, Tanaka T, Terkawi MA, Lee EG, Goo YK, Aboge GO, Yamagishi J, Hayashi K, Kameyama K, Cao S, Nishikawa Y, Xuan X.
TgGRA23, a novel Toxoplasma gondii dense granule protein associated with the parasitophorous vacuole membrane and intravacuolar network.
Parasitol Int. 62: 372-379. Aug (2013) pubmed
Maeda H, Boldbaatar D, Kusakisako K, Galay RL, Aung KM, Umemiya-Shirafuji R, Mochizuki M, Fujisaki K, Tanaka T.
Inhibitory effect of cyclophilin A from the hard tick Haemaphysalis longicornis on the growth of Babesia bovis and Babesia bigemina.
Parasitol Res. 112: 2207-2213. Jun (2013) pubmed
Galay RL, Aung KM, Umemiya-Shirafuji R, Maeda H, Matsuo T, Kawaguchi H, Miyoshi N, Suzuki H, Xuan X, Mochizuki M, Fujisaki K, Tanaka T.
Multiple ferritins are vital to successful blood feeding and reproduction of the hard tick Haemaphysalis longicornis.
J Exp Biol. 216:1905-1915. Feb (2013) pubmed
Yamaji K, Miyoshi T, Hatta T, Matsubayashi M, Alim MA, Anisuzzaman, Kushibiki S, Fujisaki K, Tsuji N.
HlCPL-A, a cathepsin L-like cysteine protease from the ixodid tick Haemaphysalis longicornis, modulated midgut proteolytic enzymes and their inhibitors during blood meal digestion.
Infect Genet Evol. 16C: 206-211. Feb (2013) pubmed

2012

Hatta T, Miyoshi T, Matsubayshi M, Islam MK, Alim MA, Anisuzzaman, Yamaji K, Fujisaki K, Tsuji N.
Semi-artificial mouse skin membrane feeding technique for adult tick, Haemaphysalis longicornis.
Parasit Vectors 5: 263. Nov (2012) pubmed
Umemiya-Shirafuji R, Boldbaatar D, Liao M, Battur B, Morshedur Rahman M, Harnnoi T, Galay RL, Tanaka T, Fujisaki K.
Target of rapamycin (TOR) controls vitellogenesis via activation of the S6 kinase in the fat body of the tick, Haemaphysalis longicornis.
Int J Parasitol. 42: 991-998. Oct (2012) pubmed
Alim MA, Islam MK, Anisuzzaman, Miyoshi T, Hatta T, Yamaji K, Matsubayashi M, Fujisaki K, Tsuji N
A hemocyte-derived Kunitz-BPTI-type chymotrypsin inhibitor, HlChI, from the ixodid tick Haemaphysalis longicornis, plays regulatory functions in tick blood-feeding processes.
Insect Biochem Mol Biol. 42: 925-934. Sep (2012) pubmed
Terada, Y., Shiozaki, Y., Shimoda, H., Mahmoud, H., Noguchi, K., Nagao, Y., Shimojima, M., Iwata, H., Mizuno, T., Okuda, M., Morimoto, M., Hayashi, T., Tanaka, Y., Mochizuki, M., Maeda, K.
Feline infectious peritonitis virus with a large deletion in the 5' terminal region of spike gene retains its virulence for cats.
J Gen Virol. 93: 1930-1934. Sep (2012) pubmed
Yu L, Yamagishi J, Zhang S, Jin C, Aboge GO, Zhang H, Zhang G, Tanaka T, Fujisaki K, Nishikawa Y, Xuan X.
Protective effect of a prime-boost strategy with plasmid DNA followed by recombinant adenovirus expressing TgAMA1 as vaccines against Toxoplasma gondii infection in mice.
Parasitol Int. 61: 481-486. Sep (2012) pubmed
Kume A, Boldbaatar D, Takazawa Y, Umemiya-Shirafuji R, Tanaka T, Fujisaki K.
RNAi of the translation inhibition gene 4E-BP identified from the hard tick, Haemaphysalis longicornis, affects lipid storage during the off-host starvation period of ticks.
Parasitol Res. 111: 889-896. Aug (2012) pubmed
Klionsky DJ, Fujisaki K ,Umemiya-Shirafuji R et al.
Guidelines for the use and interpretation of assays for monitoring autophagy. Autophagy 8: 445-544. Apr (2012) pubmed
Aung KM, Boldbaatar D, Umemiya-Shirafuji R, Liao M, Tsuji N, Xuenan X, Suzuki H, Kume A, Galay RL, Tanaka T, Fujisaki K.
HlSRB, a Class B scavenger receptor, is key to the granulocyte-mediated microbial phagocytosis in ticks.
PLoS One 7: e33504. Mar (2012) pubmed
Umemiya-Shirafuji R, Tanaka T, Boldbaatar D, Tanaka T, Fujisaki K
Akt is an essential player in regulating cell/organ growth at the adult stage in the hard tick Haemaphysalis longicornis
Insect Biochem Mol Biol. 42: 164-173. Mar (2012) pubmed
Tanaka T, Maeda H, Matsuo T, Boldbattar D, Umemiya-Shirafuji R, Kume A, Suzuki H, Xuan X, Tsuji N, Fujisaki K.
Parasiticidal activity of Haemaphysalis longicornis longicin P4 peptide against Toxoplasma gondii
Peptides 34: 242-250. Mar (2012) pubmed
Anisuzzaman, Islam MK, Alim MA, Miyoshi T, Hatta T, Yamaji K, Matsumoto Y, Fujisaki K, Tsuji N.
Longistatin is an unconventional serine protease and induces protective immunity against tick infestation.
Mol Biochem Parasitol. 182: 45-53. Mar-Apr (2012) pubmed
Galay RL, Maeda H, Aung KM, Umemiya-Shirafuji R, Xuan X, Igarashi I, Tsuji N, Tanaka T, Fujisaki K.
Anti-babesial activity of a potent peptide fragment derived from longicin of Haemaphysalis longicornis.
Trop Anim Health Prod. 44: 343-348. Feb (2012) pubmed

2011

Aung KM, Boldbaatar D, Umemiya-Shirafuji R, Liao M, Xuenan X, Suzuki H, Galay RL, Tanaka T, Fujisaki K.
Scavenger receptor mediates systemic RNA interference in ticks.
PLoS One 6: e28407. Dec (2011) pubmed
Kawano S, Umemiya-Shirafuji R, Boldbaatar D, Matsuoka K, Tanaka T, Fujisaki K.
Cloning and characterization of the autophagy-related gene 6 from the hard tick, Haemaphysalis longicornis.
Parasitol Res. 109: 1341-1349. Nov (2011) pubmed
Anisuzzaman, Khyrul Islam M, Abdul Alim M, Miyoshi T, Hatta T, Yamaji K, Matsumoto Y, Fujisaki K, Tsuji N.
Longistatin, a novel plasminogen activator from vector ticks, is resistant to plasminogen activator inhibitor-1.
Biochem Biophys Res Commun. 413: 599-604. Sep (2011) pubmed
Yu L, Zhang S, Liang W, Jin C, Jia L, Luo Y, Li Y, Cao S, Yamagishi J, Nishikawa Y, Kawano S, Fuujisaki K,
Xuan X.
Epidemiological survey of Theileria parasite infection of cattle in northeast China by allele-specific PCR.
J Vet Med Sci. 73: 1509-1512. Jul (2011) pubmed
Zhang H, Lee EG, Yu L, Kawano S, Huang P, Liao M, Kawase O, Zhang G, Zhou J, Fujisaki K, Nishikawa Y,
Xuan X.
Identification of the cross-reactive and species-specific antigens between Neospora caninum and Toxoplasma gondii tachyzoites by proteomics approach.
Parasitol Res. 109: 899-911. Apr (2011) pubmed
Sassa Y, Yamamoto H, Mochizuki M, Umemura T, Horiuchi M, Ishiguro N, Miyazawa T.
Successive death of a captive snow leopard (Uncia uncia) and a serval (Leptailurus serval) by infection with feline panleukopenia virus.
J Vet Med Sci. 73: 491-494. Apr (2011) pubmed
Anisuzzaman, Islam MK, Alim MA, Miyoshi T, Hatta T, Yamaji K, Matsumoto Y, Fujisaki K, Tsuji N.
Longistatin, a plasminogen activator, is key to the availability of blood-meals for ixodid ticks.
PLoS Pathog. 7: e1001312. Mar (2011) pubmed
Yamagishi J, Wakaguri H, Sugano S, Kawano S, Fujisaki K, Sugimoto C, Watanabe J, Suzuki Y, Kimata I, Xuan X.
Construction and analysis of full-length cDNA library of Cryptosporidium parvum.
Parasitol Int. 60: 199-202. Mar (2011) pubmed
Aung KM, Boldbaatar D, Liao M, Umemiya-Shirafuji R, Nakao S, Matsuoka T, Tanaka T, Fujisaki K.
Identification and characterization of class B scavenger receptor CD36 from the hard tick, Haemaphysalis longicornis.
Parasitol Res.108: :273-285. Feb (2011) pubmed
Luo Y, Jia H, Terkawi MA, Goo YK, Kawano S, Ooka H, Li Y, Yu L, Cao S, Yamagishi J, Fujisaki K, NishikawaY,Saito-Ito A, Igarashi I, Xuan X.
Identification and characterization of a novel secreted antigen 1 of Babesia microti and evaluation of its potential use in enzyme-linked immunosorbent assay and immunochromatographic test.
Parasitol Int. 60: 119-125. Jan (2011) pubmed

2010

Tanaka T, Kawano S, Nakao S, Umemiya-Shirafuji R, Rahman MM, Boldbaatar D, Battur B, Liao M, Fujisaki K.
The identification and characterization of lysozyme from the hard tick Heamaphysalis longicornis.
Ticks Tick Borne Dis. 1: 178-185. Dec (2010) pubmed
Boldbaatar D, Umemiya-Shirafuji R, Liao M, Tanaka T, Xuan X, Fujisaki K.
Multiple vitellogenins from the Haemaphysalis longicornis tick are crucial for ovarian development.
J Insect Physiol. 56: 1587-1598. Nov (2010) pubmed
Ohshima T, Kawakami K, Abe T, Mochizuki M.
A Minute Virus of Canines (MVC: Canine Bocavirus) Isolated from an Elderly Dog with Severe Gastroenteritis, and Phylogenetic Analysis of MVC Strains.
Vet Microbiol. 145: 334-338. Oct (2010) pubmed
Boldbaatar D, Umemiya-Shirafuji R, Liao M, Tanaka T, Xuan X, Fujisaki K.
Multiple vitellogenins from the Haemaphysalis longicornis tick are crucial for ovarian development.
J Insect Physiol. 56: 1587-1598. Sep (2010) pubmed
Aung KM, Boldbaatar D, Liao M, Umemiya-Shirafuji R, Nakao S, Matsuoka T, Tanaka T, Fujisaki K.
Identification and characterization of class B scavenger receptor CD36 from the hard tick, Haemaphysalis longicornis.
Parasitol Res. 108: 273-285. Sep (2010) pubmed
Zhou J, Liao M, Gong H, Xuan X, Fujisaki K.
Characterization of Hlcyst-3 as a member of cystatins from the tick Haemaphysalis longicornis.
Exp Appl Acarol. 51: 327-333. Aug (2010) pubmed
Malagoli D, Abdalla FC, Cao Y, Feng Q, Fujisaki K, Gregorc A, Matsuo T, Nezis IP, Papassideri IS, Sass M, Silva-Zacarin EC, Tettamanti G, Umemiya-Shirafuji R.
Autophagy and its physiological relevance in arthropods: Current knowledge and perspectives.
Autophagy 6: 575-588. Jul (2010) pubmed
Shimoda H, Ohno Y, Mochizuki M, Iwata H, Okuda M, Maeda K.
Dogs as Sentinels for Human Infection with Japanese Encephalitis Virus.
Emerg Infect Dis. 16: 1137-1139. Jul (2010) pubmed
Hatta T, Tsuji N, Miyoshi T, Islam MK, Alim MA, Yamaji K, Anisuzzaman, Fujisaki K.
Leucine aminopeptidase, HlLAP, from the ixodid tick Haemaphysalis longicornis, plays vital roles in the development of oocytes.
Parasitol Int. 59: 286-289. Jun (2010) pubmed
Tanaka T, Rahman MM, Battur B, Boldbaatar D, Liao M, Umemiya-Shirafuji R, Xuan X, Fujisaki K.
Parasiticidal activity of human alpha-defensin-5 against Toxoplasma gondii.
In Vitro Cell Dev Biol Anim. 46: 560-565. Jun (2010) pubmed
Umemiya-Shirafuji R, Matsuo T, Liao M, Boldbaatar D, Battur B, Suzuki H, Fujisaki K.
Increased expression of ATG genes during nonfeeding periods in the tick Haemaphysalis longicornis.
Autophagy 6: 473-481. May (2010) pubmed
Yamaji K, Tsuji N, Miyoshi T, Hatta T, Alim MA, Anisuzzaman, Kushibiki S, Fujisaki K.
Hlcyst-1 and Hlcyst-2 are potential inhibitors of HlCPL-A in the midgut of the Ixodid tick Haemaphysalis longicornis.
J Vet Med Sci. 72: 599-604. May (2010) pubmed
Anisuzzaman, Islam MK, Miyoshi T, Alim MA, Hatta T, Yamaji K, Matsumoto Y, Fujisaki K, Tsuji N.
Longistatin, a novel EF-hand protein from the ixodid tick Haemaphysalis longicornis, is required for acquisition of host blood-meals.
Int J Parasitol. 40: 721-729. May (2010) pubmed
Miyoshi T, Tsuji N, Islam MK, Alim MA, Hatta T, Yamaji K, Anisuzzaman, Fujisaki K.
A Kunitz-type proteinase inhibitor from the midgut of the ixodid tick, Haemaphysalis longicornis, and its endogenous target serine proteinase.
Mol Biochem Parasitol. 170: 112-115. Apr (2010) pubmed
Kawakami K, Ogawa H, Maeda K, Imai A, Ohashi E, Matsunaga S, Tohya Y, Ohshima T, Mochizuki M.
A nosocomial outbreak of serious canine infectious tracheobronchitis (Kennel cough) caused by canine herpesvirus infection.
J Clin Microbiol. 48: 1176-81. Apr (2010) pubmed
Rahman M, Tsuji N, Boldbaatar D, Battur B, Liao M, Umemiya-Shirafuji R, You M, Tanaka T, Fujisaki K.
Structural characterization and cytolytic activity of a potent antimicrobial motif in longicin, a defensin-like peptide in the tick Haemaphysalis longicornis.
J Vet Med Sci. 72: 149-156. Feb (2010) pubmed
Boldbaatar D, Battur B, Umemiya-Shirafuji R, Liao M, Tanaka T, Fujisaki K.
GATA transcription, translation and regulation in Haemaphysalis longicornis tick: Analysis of the cDNA and an essential role for vitellogenesis.
Insect Biochem Mol Biol. 40: 49-57. Jan (2010) pubmed
Samman A, Logan N, McMonagle EL, Ishida T, Mochizuki M, Willett BJ, Hosie MJ.
Neutralization of Feline Immunodeficiency Virus by Antibodies Targeting the V5 Loop of Env.
J Gen Virol. 91: 242-249. Jan (2010) pubmed

外部資金獲得状況（2010年度以降）

研究組織代表者の外部資金のみを示し、分担研究の科研費などの資金は除く

国または地方公共団体等からの研究費

オウシマダニ防圧へ向けた抗酸化分子を標的とするRNA殺ダニ剤の開発
二国間交流事業
田仲哲也ら
期間：2021年度～2022年度
組換えアクアポリンとフェリチンのクリイロコイタマダニに対するワクチン評価
二国間交流事業
田仲哲也ら
期間：2020年度～2022年度

研究成果展開事業 [研究成果最適展開支援プログラム(A-STEP)]

新しい遺伝子検出技術を用いたマダニ媒介性動物感染症の簡易･迅速診断方法の開発
A-STEPトライアウトタイプ
田仲哲也ら
期間：2021年度
マダニの生物活性分子(TBM)を用いた難治性原虫病の予防・治療開発
フィージビリティスタディ(FS)ステージ探索タイプ
田仲哲也ら
期間：2012年度～2013年度

日本学術振興会科学研究費補助金

二本鎖RNAバイオナノカプセルを用いた抗酸化分子を標的とするRNA殺ダニ剤の開発
基盤研究(B)
田仲哲也ら
期間：2022年度～2024年度
フィリピンにおける牛マダニ媒介性感染症のゲノム疫学調査と実践的診断法の構築
国際共同研究強化(B)
田仲哲也ら
期間：2020年度～2023年度
病原体伝播を阻止するマダニの遺伝子改変ドライブの開発
挑戦的研究(萌芽)
田仲哲也
期間：2017年度～2018年度
マダニ制圧へ向けたマダニの抗酸化分子を標的とするワクチンとRNA殺ダニ剤の創出
基盤研究(B)
田仲哲也ら
期間：2016年度～2018年度
マダニ過酸化水素消去酵素に着目した高免疫効率型の新規抗マダニワクチン開発への挑戦
特別研究員奨励費
草木迫浩大、田仲哲也
期間：2016年度～2017年度
フィリピンにおける牛のマダニ媒介性動物感染症の実態解明と診断・予防法の確立
基盤研究(B)
田仲哲也ら
期間：2015年度～2018年度
人工ヌクレアーゼTALENを用いた遺伝子ノックアウトマダニ感染症モデルの作出
挑戦的萌芽
田仲哲也
期間：2014年度～2015年度
マダニの自然免疫関連膜受容体を標的としたバベシア原虫媒介阻止ワクチンの開発
特別研究員奨励費
前田大輝、田仲哲也
期間：2014年度～2016年度
Toll情報伝達経路を標的とした新規のマダニ防圧法開発
基盤研究(B)
田仲哲也ら
期間：2013年度～2016年度
牛乳中の高機能タンパク質の組換えウイルスを用いた新規のマダニ防圧法開発
基盤研究(C)
田仲哲也ら
期間：2010年度～2012年度

特許

出願番号: 特許出願2018-197158、出願日：2018年10月19日、発明の名所: マラリア原虫増殖阻害用組成物、出願人: 鹿児島大学、発明者: 宮田健・田仲哲也・大林桃百香、代理人: 弁理士: 平木祐輔
出願番号：特許出願2016-145509、出願日：2016年7月25日、発明の名称：抗微生物ポリペプチド、出願人：鹿児島大学、発明者：田仲哲也・メルボルンリオタラクタック・藤崎幸蔵、代理人：弁理士平木祐輔
出願番号：特許出願2014-039192、出願日：2014年2月28日、発明の名称：抗マダニワクチン、出願人：鹿児島大学、発明者：田仲哲也・ガライレミルリンガートン・藤崎幸蔵、代理人：弁理士平木祐輔